Phospholipids in N. crassa

The extraction of inositol-containing phospholipids and phosphatidylcholine from Saccharomyces cerevisiae and Neurospora crassa.

By use of fungi grown in the presence of [3H]-inositol and [14C]choline, we have explored methods for the quantitative extraction of inositol-containing phospholipids and phosphatidylcholine. Slightly alkaline mixtures of both ethanol-water and ethanol-diethylether-water at elevated temperatures were shown to effectively extract these lipids from intact Saccharomyces cerevisiae and Neurospora c...

Deacetylation of N-acetyl-L-glutamic acid by Neurospora crassa.

Photosynthetic bacteria and blue-green algae synthesize ornithine from glutamic acid by a cyclic route in which the intermediate N-acetylglutamic acid is regenerated by a transacetylation reaction; furthermore, in these microorganisms, arginine, which is synthesized from ornithine, is a specific inhibitor of N-acetyl-,y-glutamokinase which phosphorylates N-acetylglutamic acid on its y-carboxyl ...

THE PHOSPHOLIPIDS OF SHEEP THYROID By N. FREINKEL*

There is much evidence for the importance of phosphorus in the metabolism and function of the thyroid gland. Kocher (1902) first described an inverse relationship between the contents of iodine and phosphorus in this tissue. More recently, Dempsey & Singer (1946) have presented histochemical evidence for marked fluctuations in the activities of acid and alkaline phosphatases and of hexose phosp...

Modification of the Neurospora crassa plasma membrane [H+]-ATPase with N,N'-dicyclohexylcarbodiimide.

The [H+]-ATPase of the Neurospora plasma membrane is composed of a single Mr = 104,000 polypeptide (B. J. Bowman, F. Blasco, and C. W. Slayman, J. Biol. Chem. (1981) 256, 12343-12349). The carboxyl-modifying reagent N,N'-dicyclohexylcarbodiimide (DCCD) inactivates the ATPase with pseudo-first order kinetics, suggesting that one site on the enzyme is involved. The rate constant for inactivation ...

Quelling in Neurospora crassa.